2 Substrate Specificity of MMPs

Alteration of substrate specificity of rat neurolysin from matrix metalloproteinase-2/9-type to -3-type specificity by comprehensive mutation.

The substrate specificity of rat brain neurolysin was rapidly modified by semirational mutagenesis coupled with a yeast molecular display system. Neurolysin mainly recognizes substrates with sequential six residues close to the scissile bond in polypeptides, cleaving a peptide bond in the center position of the six residues. To alter the recognition of the P2' amino acid of substrates by neurol...

Substrate Specificity of MMPs

The Structural Biology and Critical Evaluation of Bacterial Proteases as Targets in New Drug Design

Bacteria produce a range of proteolytic enzymes (for which a number human equivalent or structurally similar examples exist) and the primary focus of this study was to analyse the published literature to find proteolytic enzymes, specifically endoproteses and to examine the similarity in the substrates that they act on so as to predict a suitable structural motif which can be used as the basis ...

Comparative study of matrix metalloproteinase expression between African American and Caucasian Women

To date there are 26 human matrix metalloproteinases (MMPs) which are classified according to their substrate specificity and structural similarities. The four major subgroups of MMPs are gelatinases, interstitial collagenases, stromelysins, and membrane-type matrix metalloproteinases (MT-MMPs). This study investigates the expression of 26 MMPs, which have been shown to play a role in cancer me...

Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis

Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases with an extensive range of substrate specificities. Collectively, these enzymes are able to degrade various components of extracellular matrix (ECM) proteins. Based on their structure and substrate specificity, they can be categorized into five main subgroups, namely (1) collagenases (MMP-1, MMP-8 and MMP-13); (2) gelatinases (...